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Pavan Kumar, Padarthi
- Evaluation of Cytotoxicity and Acute Oral Toxicity Of Two Anthraquinones
Abstract Views :97 |
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Authors
Pottella Srinivasulu
1,
Padarthi Pavan Kumar
2,
Ch. Aruna Kumar
2,
S. Vidyadhara
3,
Janga Ramesh Babu
3
Affiliations
1 Acharya Nagarjuna University, Guntur – 522510, Andhra Pradesh, IN
2 Department of Physiology and Pharmacology, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, IN
3 3Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh, IN
1 Acharya Nagarjuna University, Guntur – 522510, Andhra Pradesh, IN
2 Department of Physiology and Pharmacology, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, IN
3 3Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 27, No 3&4 (2020), Pagination: 125-133Abstract
Bioactive compounds have been a significant therapeutic agent for decades, but the use of bio-active natural metabolites in traditional medicines and the discovery of drugs are still active. Anthraquinones are one of the secondary metabolites that are responsible for colour, fragrance and flavour to the plant; also used as colouring agents in the food and textile industries. The present aim of the study was to evaluate the cytotoxicity of Quinizarine (QNZ) and 2-Methyl Anthraquinone (MAQ) in C8-B4 (microglia cell lines, Mouse), SH-SY-5Y (Neuroblastoma cell lines, Human). As well, the study extended to determine the acute toxicity of these compounds on rat Sprague Dawley. Inhibitory concentration (IC50) was determined for both compounds as well as acute toxicity of MAQ was determined in Sprague Dawley (SD) rat. The IC50 values of MAQ and QNZ were 88.66 μg/ml, 55.33 μg/ml on C8-B4 and 88.68 μg/ml, 108.89 μg/ml on SH-SY-5Y, respectively. The acute toxicity study of fixed doses was carried out in female SD rats for both compounds. The LD50 of QNZ and MAQ were found to be greater than 5000 mg/kg and less than 300 mg/kg, respectively. The haematological investigation did not show any significant variation changes among treated rats. The following biochemical parameters were investigated to include Kidney Function Tests (KFTs), Liver Function Tests (LFTs), lipid profile, electrolytes and Random Blood Glucose (RBG) were found no significant changes in the QNZ treated group as compared with the sham group but elevation of Na+, creatinine and SGOT levels in MAQ treated animals. Gross necropsy showed non-significant alteration upon QNZ administration but Lung inflammation was found in the MAQ group. The histopathological finding suggested no significant alteration in tissue histology in QNZ group but infiltration of neutrophils, lymphocytes and macrophages in MAQ treated group. A high dose of both compounds for Single oral administration did not produce any significant alteration in morphological and behavioural parameters. The histopathological finding also supports the safety of both compounds in SD Female rats.Keywords
Acute Toxicity, C8-B4, Quinizarine, SH-SY-5Y, 2-methyl Anthraquinone
References
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- Evaluation of the Antibacterial Activity of Isolated Anthraquinones from the Root of Rubia Cordifolia Linn
Abstract Views :85 |
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Authors
Pottella Srinivasulu
1,
Padarthi Pavan Kumar
2,
Ch Aruna Kumar
2,
J. Venkateswara Rao
3,
S. Vidyadhara
3,
Janga Ramesh Babu
3
Affiliations
1 Acharya Nagarjuna University, Guntur – 522510, Andhra Pradesh,, IN
2 Department of Physiology and Pharmacology, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, IN
3 Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, IN
1 Acharya Nagarjuna University, Guntur – 522510, Andhra Pradesh,, IN
2 Department of Physiology and Pharmacology, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, IN
3 Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 27, No 3&4 (2020), Pagination: 193-201Abstract
The present study was intended to screen the antibacterial activity of anthraquinones i.e. 2-Methyl Anthraquinone (MAQ) and Quinizarine (QNZ), isolated from roots of Rubia cordifolia Linn. Minimum Inhibitory Concentration (MIC) of isolated anthraquinones was evaluated against selected gram-positive [Bacillus subtilis (BS) and Staphylococcus aureus (SA)] and gram-negative [Serratia marcescens (SM) and Escherichia coli (EC)] bacterial strains. The MIC was determined by micro broth dilution method using Tryptone Soya Broth (TSB) in 96 well plates as well as by measuring zone of inhibition using nutrient agar medium by the cup-plate method. Absorbance values after micro broth dilution revealed the significant MIC of MAQ as 40 μg/mL against BS, 40 μg/mL of MAQ against SA and 80 μg/mL of MAQ against SM. Whereas, The MIC of QNZ was 20 μg/mL against EC. Percentage zone of inhibition after cup-plate method revealed the significant MIC of MAQ as 39.8% against BS when compared with vancomycin, 88.33% against SA when compared with linezolid, 53.37% against SM when compared with amikacin. Whereas, percentage zone of inhibition revealed the significant MIC of QNZ as 53.76% against EC when compared with ciprofloxacin. Based on the MIC results after both micro broth dilution and cup-plate method, we observed that MAQ has shown considerable antibacterial activity against selected bacteria.Keywords
MIC, Micro Broth Dilution, Quinizarine, 2-Methyl Anthraquinone.References
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